Blood analysis method, control device, and blood cell analyzer

ABSTRACT

The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.

TECHNICAL FIELD

The present disclosure relates to the field of medical technology, moreparticularly to a blood analyzer, a control device and a blood analysismethod.

BRIEF SUMMARY

The present disclosure relates to methods and apparatuses for bloodanalysis. More particularly, the present disclosure relates to a bloodanalysis method for a blood analyzer, wherein a first reagent is mixedwith a sample to obtain a first testing sample for leukocyteclassification, and then a second reagent is mixed with the firsttesting sample for a further reaction to get a second testing sample forBASO classification and/or HGB measurement. Moreover, the presentdisclosure relates a control device and a blood analyzer thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

Similar reference characters in drawings refer to the same, functionallysimilar, and/or structurally similar elements. It should be understoodthat the drawings are intended to illustrate exemplary embodiments butare not intended to in any way limit the scope of the invention as setforth in the claims.

FIG. 1 is a leukocyte detection flowchart in accordance with oneembodiment of the present disclosure.

FIG. 2 is a schematic diagram of leukocyte detection in accordance withone embodiment of the present disclosure.

FIG. 3 is schematic diagram of leukocyte detection in accordance withanother embodiment of the present disclosure.

FIG. 4 is structural schematic diagram of a flow cytometer in accordancewith one embodiment of the present disclosure.

FIG. 5 is a leukocyte detection flowchart in accordance with anotherembodiment of the present disclosure in accordance with anotherembodiment of the present disclosure.

FIG. 6 is a schematic diagram of leukocyte detection in accordance withone embodiment of the present disclosure.

FIG. 7 is schematic diagram of leukocyte detection in accordance withanother embodiment of the present disclosure.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

A blood analyzer is used to analyze components in a blood sample, forexample counting and classifying leukocytes in the blood sample,measuring hemoglobin (HGB) concentration and so on. Leukocytes in humanblood have five groups: lymphocyte, monocyte, neutrophil, basophil andeosinophil, which can be counting and classified into these five groupsby the blood analyzer.

Now, there are two methods for classifying leukocytes into five groups.One is single-channel method and the other is two-channel method. Insingle-channel method, there is only one detection channel for leukocyteclassification, and a blood sample reacts with a reagent in one reactioncell to obtain a testing sample, which is transferred to a detectionapparatus for obtaining five-group classification information by singledetection. In two-channel method, there are one DIFF channel for cellcounting & differentiation and one independent BASO channel for basophildetection only. One blood sample is divided into two aliquots, and thesetwo aliquots are reacted with reagents in two reaction cells anddetected by two channels respectively, and leukocyte four-groupclassification result (ie. Leukocytes are classified into four groups,lymphocyte group, monocyte group, neutrophil & basophil group andeosinophil group) is obtained by DIFF channel, and basophil result isobtained by BASO channel and then these two results are combined toobtained five-group classification result.

The single-channel method might have unsatisfied reliability because itmight be difficult to differentiate basophils from other cells ofleukocytes when other cells are not damaged. In addition, a bloodanalyzer using the single-channel method might still have independentchannel to meet requirement of HGB measurement. So, this kind ofanalyzer might still have two channels and/or two reaction cells for tworeactions.

The two-channel method might need more blood sample amount and morekinds of regents because of two independent reactions. And this methodmight have unsatisfied test speed, because two detections might becarried out in sequence. Moreover, a blood analyzer using this methodmight have more complex structure and larger size because it has tworeaction cells, two sets of sample transferring apparatuses.

Therefore, there might be need to continue to develop a low cost andmore accurate blood analysis method, control device and blood analyzer.

The scope of the present invention is defined solely by the appendedclaims, and is not affected to any degree by the statements within thissummary.

In one aspect of the present disclosure, there is provided a bloodanalysis method for a blood cell analyzer, the blood cell analyzercomprising a reaction cell where a testing sample is prepared from ablood sample, and a detection apparatus which detects the testing samplefor leukocyte detection, and the method comprising:

injecting a blood sample and a first reagent into the reaction cell,wherein the first reagent lyses erythrocytes in the blood sample andreacts with leukocytes in the blood sample to obtain a first testingsample;

transferring a part of the first testing sample from the reaction cellto the detection apparatus for leukocyte four-group classificationdetection;

injecting a second reagent into the reaction cell after transferring thepart of the first testing sample, wherein the second reagent reacts withleukocytes in a remainder of the first testing sample to obtain a secondtesting sample; and

transferring the second testing sample to the detection apparatus forbasophil classification detection.

In another aspect of the present disclosure, there is provided a bloodanalysis method for a blood cell analyzer, the blood cell analyzercomprising a reaction cell where a testing sample is prepared from ablood sample, and a detection apparatus which detects the testingsample, and the method comprising:

injecting a blood sample and a first reagent in the reaction cell,wherein the first reagent lyses erythrocytes in the blood sample andreacts with leukocytes in the blood sample to obtain a first testingsample;

transferring a part of the first testing sample from the reaction cellto the detection apparatus for leukocyte five-group classificationdetection;

injecting a second reagent into the reaction cell after transferring thepart of the first testing sample, wherein the second reagent reacts withleukocytes in a remainder of the first testing sample to obtain a secondtesting sample; and

measuring the second testing sample for hemoglobin measurement.

In still another aspect of the present disclosure, there is provided acontrol device for a blood cell analyzer, the blood cell analyzercomprising a reaction cell where a testing sample is prepared from ablood sample, and a detection apparatus which detects the testingsample, and the control device comprising:

a first control unit, which controls a sample injecting apparatus toinject a blood sample into the reaction cell, controls a reagentinjecting apparatus to inject a first reagent for leukocyteclassification into the reaction cell, wherein the first reagent lyseserythrocytes in the blood sample and reacts with leukocytes in the bloodsample to obtain a first testing sample;

a second control unit, which controls a transferring apparatus totransfer a part of the first testing sample from the reaction cell tothe detection apparatus for leukocyte four-group classificationdetection, after the reaction between the blood sample and the firstreagent is finished;

a third control unit, which controls the reagent injecting apparatus toinject a second reagent into the reaction cell having a reminder of thefirst testing sample after the part of the first testing sample istransferred, wherein the reminder of the first testing sample reactswith the second reagent to obtain a second testing sample; and

a fourth control unit, which controls the transferring apparatus totransfer the second testing sample to the detection apparatus forbasophil classification detection when both leukocyte four-groupclassification detection and the reaction between the reminder of thefirst sample and the second reagent are finished.

In still another aspect of the present disclosure, there is provided acontrol device for a blood cell analyzer, the blood cell analyzercomprising a reaction cell where a testing sample is prepared from ablood sample, and a detection apparatus which detects the testingsample, and said control device comprising:

a first control unit, which controls a sample injecting apparatus toinject a blood sample into the reaction cell, controls a reagentinjecting apparatus to inject a first reagent for leukocyteclassification into the reaction cell, wherein the first reagent lyseserythrocytes in the blood sample and reacts with leukocytes in the bloodsample to obtain a first testing sample;

a second control unit, which controls a transferring apparatus totransfer a part of the first testing sample from the reaction cell tothe detection apparatus for leukocyte five-group classificationdetection, after the reaction between the blood sample and the firstreagent is finished;

a third control unit, which controls the reagent injecting apparatus toinject a second reagent into the reaction cell having a reminder of thefirst testing sample after the part of the first testing sample istransferred, wherein the reminder of the first testing sample reactswith the second reagent to obtain a second testing sample; and

a fifth control unit, which controls a hemoglobin measurement apparatusto measure hemoglobin while the reminder of first testing sample reactswith the second reagent.

In still another aspect of the present disclosure, there is provided ablood analyzer which comprising:

-   -   a reaction cell, where a testing sample is prepared from a blood        sample;    -   a detection apparatus, which detects the testing sample for        leukocyte detection;    -   a sample injecting apparatus, which injects the blood sample        into the reaction cell;    -   a reagent injecting apparatus, which injects a reagent into the        reaction cell;    -   a transferring apparatus, which transfers the testing sample        from the reaction cell to the detection apparatus;    -   a control device, which couples to the sample injecting        apparatus, the reagent injecting apparatus and the transferring        apparatus respectively;    -   wherein

the control device controls the sample injecting apparatus to inject ablood sample into the reaction cell, controls the reagent injectingapparatus to inject a first reagent for leukocyte classification intothe reaction cell, wherein the first reagent lyses erythrocytes in theblood sample and reacts with leukocytes in the blood sample to obtain afirst testing sample;

the control device controls the transferring apparatus to transfer apart of the first testing sample from the reaction cell to the detectionapparatus for a leukocyte classification detection after the reactionbetween the blood sample and the first reagent is finished;

the control device controls the reagent injecting apparatus to inject asecond reagent into the reaction cell having a reminder of the firsttesting sample after the part of the first testing sample istransferred, wherein the reminder of the first testing sample reactswith the second reagent to obtain a second testing sample; and

the control device controls the transferring apparatus to transfer thesecond testing sample to the detection apparatus or a hemoglobinmeasurement apparatus after the leukocyte classification detection isfinished.

In above analyzers, devices and methods, it is disclosed and utilizedthat a testing sample for leukocyte classification could be further usedfor basophil classification. After a first reagent reacts with a bloodsample to obtain a testing sample for leukocyte detection, a secondreagent is added for further reaction to obtain a testing sample forbasophil classification or HGB measurement. Therefore, a blood samplemay be tested in one reaction cell through time-division multiplexingtechnology to obtain leukocyte five-group classification result and HGBresult by single measurement channel. Thus, the structure of theanalyzer may be greatly simplified on the premise of guaranteeing theperformance of the analyzer, the volume and cost of the analyzer mayreduce and a price-performance ratio of the analyzer may increase.

The following description provides specific details for completelyunderstanding and practicing of embodiments by one skilled in the art.However, those skilled in the art will understand, the embodiment couldbe practice without some of these details. In order to avoidunnecessarily obscuring descriptions of the embodiments, some ofwell-known structures and functions are not shown or described in detailin some embodiments. Unless the context clearly requires, otherwise,throughout the specification and claims, the term “comprising”,“including” and the like should be inclusive rather than to explain themeaning of an exclusive or exhaustive sense, that is, which means“including, but not limited to”. In this detailed description section,asingular or plural terms also include the plural number or singularnumber.

In one embodiment, a blood analyzer includes one set of leukocytedetection channel, which executes two reactions and detections. There issingle reaction cell where a testing sample is prepared from a bloodsample, single detection apparatus which detects the testing sample forleukocyte detection, a sample injecting apparatus which injects theblood sample into the reaction cell, a reagent injecting apparatus whichinjects a reagent into the reaction cell, a transferring apparatus and acontrol device which transfers the testing sample from the reaction cellto the detection apparatus. The control device couples to the sampleinjecting apparatus, the reagent injecting apparatus and thetransferring apparatus respectively. And a leukocyte detection flowchartis shown in FIG. 1, which includes following steps:

step 1001, the control device controls the sample injecting apparatus toinject an amount of the blood sample sufficient for leukocyte detectionand basophil detection, and control the reagent injecting apparatus toinject a first reagent for leukocyte classification into the reactionrespectively. In the embodiment, the first reagent lyses erythrocytes inthe blood sample and reacts with leukocytes so as to make each subclassof leukocytes cluster. The first reagent reacts with the blood sample toobtain a first testing sample.

step 1002, the control device controls the transferring apparatus totransfer a part of the first testing sample from the reaction cell tothe detection apparatus after the reaction between the blood sample andthe first reagent is finished.

step 1003 a, the detection apparatus detects the first testing samplewhen it passes through the detection apparatus, which is named as afirst detection. The first detection is leukocyte four-groupclassification detection, which means that leukocytes are classifiedinto lymphocyte group, monocyte group, neutrophil & basophil group andeosinophil group and counted respectively based on signals of thedetection. After the part of the first testing sample is drawn from thereaction cell, step 1003 b is executed. The control device controls thereagent injecting apparatus to inject a second reagent into the reactioncell having a reminder of the first testing sample, and the reminder ofthe first testing sample reacts with the second reagent to obtain asecond testing sample. The second reagent may shrink the other cellsexcept for basophils. The leukocyte classification detection andreaction of the second reagent may begin at the same time;alternatively, one of them may begin firstly, and some of their processmay overlap to reduce whole time cost of leukocyte detection.

step 1004, the control device controls the transferring apparatus totransfer a suitable amount of the second testing sample from thereaction cell to the detection apparatus when both leukocyte four-groupclassification detection and reaction of the second reagent arefinished.

step 1005, the detection apparatus detects the first testing sample whenit passes through the detection apparatus, which is named as a seconddetection. The second detection is basophil classification detection,which means that basophils are classified from other cells of leukocytesand counted based on signals of the detection.

When hemoglobin of the blood sample needs to be measured, the secondtesting sample could be detected for hemoglobin measurement. Moreover,leukocyte counting and classification may be executed simultaneously; inanother word, a signal acquired in leukocyte classification detectioncould be used in leukocyte counting, because difference betweensubgroups of leukocyte does not influence leukocyte counting.Alternatively, leukocyte counting may be executed based on basophilclassification result. Schematic diagrams of leukocyte counting andhemoglobin measurement are shown in FIG. 2 and FIG. 3.

In the embodiment, components or concentrations of the first reagent andsecond reagent may be irrelative. The first reagent may be singlereagent or multi-component reagent whose components are injected intothe reaction cell according to a set sequence. The blood sample reactswith the first reagent to obtain the first testing sample. Differentfirst testing sample may be obtained according to different firstreagent. In like manner, the second reagent may be single reagent ormulti-component reagent. Any reagent which can be used for leukocytefour-group or five-group classification could be named as a firstreagent, and any reagent which can be used for basophil classificationcould be used as a second reagent.

In one embodiment, the transferring apparatus includes a syringe and atransfer pipeline which connects to the syringe, and the syringe, atesting sample outlet of the reaction cell and an inlet of the detectionapparatus are connected by the transfer pipeline. There is a cleaningprocess after the leukocyte classification is finished and before thebasophil classification is started, wherein the transfer pipeline andthe detection apparatus though which the first testing sample has passedare cleaned to prevent the first testing sample from contaminating thesecond testing sample.

In the embodiment, leukocytes are classified as follows after the bloodsample is tested:

Lymphocyte (LYM) 23.8% Monocyte (MON) 6.7% Neutrophil (NEU) 65.4%Eosinophil (ESO) 3.4% Basophil (BASO) 0.7%

Before the sample is tested, the same sample is examined by aconventional microscopy and the result is obtained as follows:

Lymphocyte (LYM) 24%  Monocyte (MON) 7% Neutrophil (NEU) 65%  Eosinophil(ESO) 3% Basophil (BASO) 1%

By comparing these two results, it is shown that the result of thepresent disclosure corresponds with that of conventional microscopy, andthe present disclosure has satisfying accuracy and reliability.

In above method for a blood cell analyzer, two reactions and twodetections are executed by single set leukocyte detection channel. Thefirst reaction is executed by mixing the blood sample and a reagent forleukocyte classification, and the second reaction is executed by mixingthe first testing sample and a reagent for basophil classification.These two reactions may be executed simultaneously. The first detectionis leukocyte four-group classification detection and the seconddetection is basophil classification detection. After two detections,the results of leukocyte four-group classification and basophilclassification are obtained, whose accuracy and reliability could meetrequirement. So the method of present disclosure retains advantages oftwo-channel method. In further, since there is single set leukocytedetection channel in the blood analyzer of the present disclosure, onereaction cell and its correlative injecting, cleaning, discharging, andmixing pipeline may be saved, so that the analyzer may has more simplestructure, smaller size and less manufacturing cost in the premise ofensuring analyzer performance. Thus the analyzer has betterperformance-price ratio. In addition, the blood sample needs to be usedin two detection channels in conventional two-channel method. However,the first testing sample is used for further reaction in the method ofthe present disclosure, so volume of blood sample may decrease andutilization rate of the sample may be improved.

Light scattering theory is usually used in leukocyte classification.Based on light scattering principle, a blood sample usually undergoes asampling process, reaction process, sample-preparing process, detectionprocess and cleaning process in a blood analyzer after it is acquired.In one embodiment, a blood analyzer has suitable pipelines and valves,and then two kinds of reagent can be injected into single reaction celland a testing sample can be transferred successively to a detectionapparatus for blood analysis. In another embodiment, as shown in FIG. 4,a blood analyzer includes a reaction cell 4, a detection apparatus 3, asample injecting apparatus, a reagent injecting apparatus, atransferring apparatus, a cleaning pipeline 203 and a control device(not shown in the drawing).

The sample injecting apparatus injects a blood sample into a reactioncell. In the embodiment, the sample injecting apparatus is sample probe6. In other embodiments, the sample injecting apparatus may have otherstructure.

The reagent injecting apparatus injects a reagent into a reaction cell.In the embodiment, the reagent injecting apparatus has two sets ofinjecting apparatus, such as a first reagent injecting apparatus 1 and asecond reagent injecting apparatus 2. The first reagent injectingapparatus 1 and the second reagent injecting apparatus 2 may be aconstant flow pump, a pressure source or a syringe. The first reagentinjecting apparatus 1 is connected with the reaction cell 4 through asixth controllable valve 16, and draws a certain amount of reagent froma reagent container 8. In another embodiment, based on user'srequirement, the first reagent injecting apparatus 1 may further includea reagent heating apparatus 7 in pipeline between the first reagentinjecting apparatus 1 and the reaction cell 4. The second reagentinjecting apparatus 2 is connected with the reaction cell 4 by a seventhcontrollable valve 17, and draws a certain amount of reagent from areagent container 9. In other embodiments, the reagent injectingapparatus may have single set injecting apparatus.

The transferring apparatus transfers a testing sample from the reactioncell to the detection apparatus. In the embodiment, the transferringapparatus includes a syringe and a transfer pipeline which connects tothe syringe. And the syringe, a testing sample outlet of the reactioncell and an inlet of the detection apparatus are connected by thetransfer pipeline, and the testing sample outlet has a firstcontrollable valve 11 for controlling “on-off” of the outlet. Thesyringe may be multiple syringes, which executes drawing and dischargingmovement based on control of the control device. A first end of thecleaning pipeline 203 connects to the transfer pipeline and the testingsample outlet 41 of the reaction cell, and a second end of the cleaningpipeline 203 connects to a waste collecting apparatus 19, and thecleaning pipeline has a second controllable valve 12 for controlling“on-off” of the cleaning pipeline. The control device couples to thefirst controllable valve 11 and the second controllable valve 12respectively for controlling their “on-off”. Controlling process of thecontrol device includes steps as follows.

The control device controls the syringe to move after the reactionbetween the blood sample and the first reagent is finished, to transfera part of a first testing sample which is prepared by a blood sample anda first reagent from the reaction cell 4 to the detection apparatus 3 bythe transfer pipeline. The control device controls the firstcontrollable valve 11 as “on” and the second controllable valve 12 as“off” during the part of the first testing sample is drawn, and controlsthe first controllable valve 11 as “off” and the second controllablevalve 12 keeping “off” after drawing of the part of the first testingsample is finished. The control device controls the first controllablevalve 11 keeping “off” and the second controllable valve 12 as “on”after the leukocyte classification detection is finished, and controlsthe syringe to draw a cleaning solution to clean the transfer pipelineand the detection apparatus 3 and discharge the cleaning solutionthrough the cleaning pipeline. The control device controls the firstcontrollable valve 11 as “on” and the second controllable valve 12 as“off” after both the cleaning and the reaction between the reminder ofthe first testing sample and the second reagent are finished, andcontrols the syringe to transfer part of the second testing samplethrough the transfer pipeline from the reaction cell 4 to the detectionapparatus 3. The control device controls the first controllable valve 11as “off” and the second controllable valve 12 keeping “off” after thedrawing of part of the second testing sample is finished.

In the embodiment, the control device includes a first control unit, asecond control unit, a third control unit and a fourth control unit. Thefirst control unit controls the sample injecting apparatus to inject anamount of blood sample sufficient for leukocyte detection and basophildetection into the reaction cell, controls the reagent injectingapparatus to inject a first reagent for leukocyte classification intothe reaction cell, where the blood sample reacts with the first reagentto obtain a first testing sample. The second control unit controls thetransferring apparatus to transfer a part of the first testing samplefrom the reaction cell to the detection apparatus for leukocytefour-group classification detection, after the reaction between theblood sample and the first reagent is finished. The third control unitcontrols the reagent injecting apparatus to inject a second reagent intothe reaction cell having a reminder of the first testing sample duringthe leukocyte four-group classification detection, wherein the reminderof the first testing sample reacts with the second reagent to obtain asecond testing sample and the second shrinks the other cells except forbasophils. The fourth control device controls the transferring apparatusto transfer the second testing sample to the detection apparatus forbasophil classification detection when both leukocyte four-groupclassification detection and reaction of the second reagent arefinished.

In the embodiment, a transferring apparatus includes a syringe 5 and atransfer pipeline which connects to the syringe. The syringe 5 includestwo syringes; one is a first syringe 51 which is a larger dischargesyringe, the other is a second syringe 52 which is a smaller dischargesyringe. The transfer pipeline includes sample acquiring pipeline 205, afirst sample preparing pipeline 201, a second sample preparing pipeline202, a second connecting pipeline 204, a first connecting pipeline 207,an auxiliary pushing pipeline 206 and a sheath fluid pipeline 208. Thefirst sample preparing pipeline 201 and the second sample preparingpipeline 202 have a first end and a second end respectively. The firstend of the first sample preparing pipeline 201 connects to a testingsample outlet 41 of the reaction cell which has the first controllablevalve 11. The second end of the first sample preparing pipeline 201connects to the first end of the second sample preparing pipeline 202.The first sample preparing pipeline 201 and the second sample preparingpipeline 202 connect to the inlet of the detection apparatus 3 by athree-way valve which is set in a junction of the first sample preparingpipeline 201 and the second sample preparing pipeline 202. The sampleacquiring pipeline 205 has a first end a second end, and the first endof the sample acquiring pipeline 205 connects to the sample probe 6. Thesecond connecting pipeline 204 has a first end and a second end, and thefirst end of the second connecting pipeline 204 connects to the secondsyringe 52, the second end of the second connecting pipeline 204connects to the second end of the sample acquiring pipeline 205 and thesecond end of the second sample preparing pipeline 202 respectively by athird controllable valve 13. The first connecting pipeline 207 has afirst end and a second end, and the first end of the first connectingpipeline 207 connects to the first syringe 51, the second end connectsto an inlet 10 for a diluent input. The auxiliary pushing pipeline 206has a first end, a second end and a fourth controllable valve 14, andthe first end of the auxiliary pushing pipeline 206 connects to thesecond connecting pipeline 204, the second end connects to the firstconnecting pipeline 207. The sheath fluid pipeline 208 has a first end,a second end and a fifth controllable valve 15, and the first end of thesheath fluid pipeline 208 connects to the first connecting pipeline 207,and the second end connects to the detection apparatus 3.

A workflow of a blood cell analyzer according to the embodiment of thepresent disclosure is as follows.

A blood sample is transferred to the reaction cell 4 by the sample probe6 under action of the syringe 5, and diluted by a diluent in a certaindilution ratio. The diluent is transferred into the reaction cell 4through a fourth controllable valve 14, the second sample preparingpipeline 202, the first sample preparing pipeline 201 and the firstcontrollable valve 11. The first reagent is transferred into thereaction cell 4 through a reagent container 8, the sixth controllablevalve 16 and the reagent heating apparatus by the first reagenttransferring apparatus 1. The first reagent reacts with the solution inthe reaction cell 4 for white blood cell (WBC) classification reactionto obtain the first testing sample. After the reaction is finished, thecontrol device controls the third controllable valve 13 to connect thesecond sample preparing pipeline 202 with the second connecting pipeline204, and the third controllable valve 13 keeps them connecting aftersampling and dividing are finished. A part of the first reactionsolution is drawn into the first sample preparing pipeline 201 and thesecond sample preparing pipeline 202 under drawing action of the syringe5 for sample preparation. Then, a second reagent is transferred into thereaction cell 4 through reagent container 9 and the seventh controllablevalve 17 by the second reagent injecting apparatus 2. The second reagentreacts with a reminder of the first testing sample in the reaction cellfor basophil (BASO) reaction to obtain a second testing sample. Andduring the BASO reaction, hemoglobin (HGB) concentration curve isrecorded, and HGB result will be obtained after the reaction becomesstable. At the same time, a diluent is being transferred through a fifthcontrollable valve 15 under pushing action of the syringe 5, and wrapsand carries the first testing sample in the second sample preparingpipeline 202 into the detection apparatus 3 for leukocyte classificationdetection. The first controllable valve 11 plays a role in blocking sothat leukocyte classification detection and BASO reaction can beexecuted simultaneously. After the leukocyte classification detectionand BASO reaction are both finished, the first sample preparing pipeline201, the second sample preparing pipeline 202 and the detectionapparatus 3 are cleaned for subsequent basophil detection. A cleaningpipeline 203 and a second controllable valve 12 are set for cleaningthose pipelines through which the sample has passed. A waste sample isdischarged into the waste collecting apparatus 19. By this design, thereaction solution for basophil detection in the reaction cell may notaffected by the cleaning process. A diluent could be transferred intothe detection apparatus 3 for cleaning through the fourth controllablevalve 14 and the fifth controllable valve 15. After cleaning process,part of the second testing sample is drawn into the first samplepreparing pipeline 201 and the second sample preparing pipeline 202under drawing action of the syringe 5 for sample preparation. Then thebasophil detection is executed in similar manner of the leukocyteclassification detection. The diluent is wrapping and carrying thesecond testing sample in the second sample preparing pipeline 202 intothe detection apparatus 3 for basophil detection, when the diluent isbeing transferred under pushing action of the syringe 5. Leukocytecounting result may be obtained by leukocyte classification detection orbasophil detection.

In the embodiment, the transferring apparatus only has one first syringeand one second syringe. The first syringe could be used in providingsheath fluid, drawing testing sample from the reaction cell or providingcleaning solution. The second syringe could be used in acquiring sampleor providing sample fluid in detection. So the syringes arerepetitive-used. And with help of suitable pipeline design, number ofcontrollable valve may be reduced and structure of fluid road may besimplified under the premise of ensuring performances of sampleacquiring, sample preparing, cell detection in flow cytometry, cleaningand etc.

In another embodiment, the first syringe and the second syringe may bejoined syringe having a common drive. The common drive makes the firstand second syringes drawing and discharging simultaneously, so one setof electric motor and drive mechanism may be saved. Before sampleacquiring, the fourth controllable valve 14 should be off status so thatthe sample probe would not be affected by the first syringe 51 when theydraw liquid. In other embodiments, the first syringe and second syringemay be driven by two independent drives.

In other embodiments, the transferring apparatus may have other fluidroad structure. The first syringe and second syringe may have singleuse. For example, the first syringe for providing sheath fluid may beindependent of that for drawing testing sample, or that of providingcleaning solution. Likewise, the second syringe for acquiring sample maybe independent of that of providing sample fluid in detection. So inthese kinds of embodiments, the transferring apparatus may have aplurality of first syringe and second syringe, and fluid road structuremay be different according to different usage of these syringes.

In still another embodiment, a first testing sample may be detected forleukocyte five-group classification and may be mixed with a secondreagent for further reaction for HGB measurement. So leukocytefive-group classification and HGB measurement may be also achieved byone channel through time-division multiplexing technology. So structureof the blood analyzer may be simplified and size may be smaller. In oneembodiment, a detection flowchart is shown in FIG. 5, which includesfollowing steps:

step 2001, the control device controls the sample injecting apparatus toinject an amount of the blood sample sufficient for leukocyte detectionand basophil detection, and control the reagent injecting apparatus toinject a first reagent for leukocyte five-group classification into thereaction respectively. The first reagent reacts with the blood sample toobtain a first testing sample.

step 2002, the control device controls a transferring apparatus totransfer a part of the first testing sample from the reaction cell tothe detection apparatus after the reaction between the blood sample andthe first reagent is finished.

step 2003 a, the detection apparatus detects the first testing samplewhen it passes through the detection apparatus, which is name as a firstdetection. The first detection is leukocyte five-group classificationdetection, which means that leukocytes are classified into lymphocytegroup, monocyte group, neutrophil group, basophil group and eosinophilgroup and respectively counted based on signals of the detection. Afterthe part of the first testing sample is transferred from the reactioncell, step 2003 b is executed. The control device controls the reagentinjecting apparatus to inject a second reagent into the reaction cellhaving a reminder of the first testing sample, and the reminder of thefirst testing sample reacts with the second reagent to obtain a secondtesting sample. The leukocyte classification detection and reaction ofthe second reagent may be started at the same time; alternatively, oneof them may begin firstly, and some of their process may overlap toreduce whole time cost of leukocyte detection.

step 2004, the control device controls a hemoglobin measurementapparatus to execute hemoglobin measurement when the first testingsample reacts with the second reagent.

In the embodiment, leukocyte counting may be executed beside hemoglobinmeasurement. The leukocyte counting may be executed based on signsacquired during leukocyte five-group classification detection, as shownin FIG. 6. Alternatively, the testing sample mixed with a second reagentmay be detected for leukocyte counting, as shown in FIG. 7.

In the embodiment, the control device includes a first control unit, asecond control unit, a third control unit and a fifth control unit. Thefirst control unit controls the sample injecting apparatus to inject anamount of blood sample sufficient for leukocyte detection and basophildetection into the reaction cell, controls the reagent injectingapparatus to inject a first reagent for leukocyte classification intothe reaction cell, wherein the first reagent reacts with leukocytes inthe blood sample to obtain a first testing sample. The second controlunit controls the transferring apparatus to transfer a part of the firsttesting sample from the reaction cell to the detection apparatus forleukocyte five-group classification detection, after the reactionbetween the blood sample and the first reagent is finished. The thirdcontrol unit controls the reagent injecting apparatus to inject a secondreagent into the reaction cell having a reminder of the first testingsample during the leukocyte classification detection, wherein thereminder of the first testing sample reacts with the second reagent toobtain a second testing sample. The fifth control unit controls thehemoglobin measurement apparatus to measure hemoglobin while thereminder of first testing sample reacts with the second reagent.

In the embodiment, the second reagent is a reagent that could react withthe first testing sample to obtain the second testing sample forhemoglobin measurement. The second reagent may be single reagent ormulti-component reagent whose components are injected into the reactioncell according to a set sequence. A reagent for hemoglobin measurementmay be the same as or different from that for basophil classification.

The blood analyzer of the present disclosure may save a set of reactioncell and pipelines thereof. Moreover, the blood analyzer may maintainthe same or similar performance of a blood analyzer using thetwo-channel method. So the blood analyzer may have betterperformance-price ratio than conventional analyzer.

The above embodiments illustrates various features of the presentdisclosure, which are described in detail, but is not intended to in anyway limit the scope of the invention as set forth in the claims. It willbe appreciated by those skilled in the art that various changes,alterations and modifications may be made to the present disclosurewithout departing from the spirit and scope of the present disclosure asclaimed. Accordingly, the scope of the present invention patentprotection should defined solely by the appended claims.

1. A blood analysis method for a blood cell analyzer, the blood cellanalyzer comprising a reaction cell where a testing sample is preparedfrom a blood sample, and a detection apparatus which detects the testingsample for leukocyte detection, and said method comprising: injecting ablood sample and a first reagent into the reaction cell, wherein thefirst reagent lyses erythrocytes in the blood sample and reacts withleukocytes in the blood sample to obtain a first testing sample;transferring a part of the first testing sample from the reaction cellto the detection apparatus for leukocyte four-group classificationdetection; injecting a second reagent into the reaction cell aftertransferring the part of the first testing sample, wherein the secondreagent reacts with leukocytes in a remainder of the first testingsample to obtain a second testing sample; and transferring the secondtesting sample to the detection apparatus for basophil classificationdetection.
 2. The method according to claim 1, wherein the reaction cellis a single reaction cell.
 3. The method according to claim 1, furthercomprising measuring the second testing sample for hemoglobinmeasurement.
 4. The method according to claim 1, further comprisingcounting leukocytes based on a signal acquired during the leukocytefour-group classification detection or a signal acquired during basophilclassification detection.
 5. The method according to claim 1, whereinthe analyzer has pipelines, and said method further comprises cleaningthe pipelines and the detection apparatus through which the firsttesting sample has passed, after the leukocyte four-group classificationdetection is finished and before the basophil classification detectionis started.
 6. A blood analysis method for a blood cell analyzer, theblood cell analyzer comprising a reaction cell where a testing sample isprepared from a blood sample, and a detection apparatus which detectsthe testing sample, and said method comprising: injecting a blood sampleand a first reagent in the reaction cell, wherein the first reagentlyses erythrocytes in the blood sample and reacts with leukocytes in theblood sample to obtain a first testing sample; transferring a part ofthe first testing sample from the reaction cell to the detectionapparatus for leukocyte five-group classification detection; injecting asecond reagent into the reaction cell after transferring the part of thefirst testing sample, wherein the second reagent reacts with leukocytesin a remainder of the first testing sample to obtain a second testingsample; and measuring the second testing sample for hemoglobinmeasurement.
 7. The method according to claim 6, further comprisingcounting leukocytes based on a signal acquired during the leukocytefive-group classification detection, or transferring a part of thesecond testing sample to the detection apparatus for leukocyte countingdetection.
 8. A control device for a blood cell analyzer, the blood cellanalyzer comprising a reaction cell where a testing sample is preparedfrom a blood sample, and a detection apparatus which detects the testingsample, and said control device comprising: a first control unit, whichcontrols a sample injecting apparatus to inject a blood sample into thereaction cell, controls a reagent injecting apparatus to inject a firstreagent for leukocyte classification into the reaction cell, wherein thefirst reagent lyses erythrocytes in the blood sample and reacts withleukocytes in the blood sample to obtain a first testing sample; asecond control unit, which controls a transferring apparatus to transfera part of the first testing sample from the reaction cell to thedetection apparatus for leukocyte four-group classification detection,after said reaction between the blood sample and the first reagent isfinished; a third control unit, which controls the reagent injectingapparatus to inject a second reagent into the reaction cell having areminder of the first testing sample after the part of the first testingsample is transferred, wherein the reminder of the first testing samplereacts with the second reagent to obtain a second testing sample; and afourth control unit, which controls the transferring apparatus totransfer the second testing sample to the detection apparatus forbasophil classification detection when both leukocyte four-groupclassification detection and said reaction between the reminder of thefirst sample and the second reagent are finished.
 9. A control devicefor a blood cell analyzer, the blood cell analyzer comprising a reactioncell where a testing sample is prepared from a blood sample, and adetection apparatus which detects the testing sample, and said controldevice comprising: a first control unit, which controls a sampleinjecting apparatus to inject a blood sample into the reaction cell,controls a reagent injecting apparatus to inject a first reagent forleukocyte classification into the reaction cell, wherein the firstreagent lyses erythrocytes in the blood sample and reacts withleukocytes in the blood sample to obtain a first testing sample; asecond control unit, which controls a transferring apparatus to transfera part of the first testing sample from the reaction cell to thedetection apparatus for leukocyte five-group classification detection,after said reaction between the blood sample and the first reagent isfinished; a third control unit, which controls the reagent injectingapparatus to inject a second reagent into the reaction cell having areminder of the first testing sample after the part of the first testingsample is transferred, wherein the reminder of the first testing samplereacts with the second reagent to obtain a second testing sample; and afifth control unit, which controls a hemoglobin measurement apparatus tomeasure hemoglobin while the reminder of first testing sample reactswith the second reagent.
 10. A blood analyzer, said blood analyzercomprising: a reaction cell, where a testing sample is prepared from ablood sample; a detection apparatus, which detects the testing samplefor leukocyte detection; a sample injecting apparatus, which injects theblood sample into the reaction cell; a reagent injecting apparatus,which injects a reagent into the reaction cell; a transferringapparatus, which transfers the testing sample from the reaction cell tothe detection apparatus; a control device, which couples to the sampleinjecting apparatus, the reagent injecting apparatus and thetransferring apparatus respectively; wherein the control device controlsthe sample injecting apparatus to inject a blood sample into thereaction cell, controls the reagent injecting apparatus to inject afirst reagent for leukocyte classification into the reaction cell,wherein the first reagent lyses erythrocytes in the blood sample andreacts with leukocytes in the blood sample to obtain a first testingsample; the control device controls the transferring apparatus totransfer a part of the first testing sample from the reaction cell tothe detection apparatus for a leukocyte classification detection aftersaid reaction between the blood sample and the first reagent isfinished; the control device controls the reagent injecting apparatus toinject a second reagent into the reaction cell having a reminder of thefirst testing sample after the part of the first testing sample istransferred, wherein the reminder of the first testing sample reactswith the second reagent to obtain a second testing sample; and thecontrol device controls the transferring apparatus to transfer thesecond testing sample to the detection apparatus or a hemoglobinmeasurement apparatus after the leukocyte classification detection isfinished.
 11. The blood analyzer according to claim 10, wherein thetransferring apparatus comprises a syringe which couples to the controldevice and a transfer pipeline which connects to the syringe; thesyringe, a testing sample outlet of the reaction cell and an inlet ofthe detection apparatus are connected by the transfer pipeline, and thetesting sample outlet has a first controllable valve for controlling“on-off” of the outlet.
 12. The blood analyzer according to claim 11,said blood analyzer further comprising a cleaning pipeline, wherein afirst end of the cleaning pipeline connects to the transfer pipeline andthe testing sample outlet of the reaction cell, and a second end of thecleaning pipeline connects to a waste collecting apparatus, and thecleaning pipeline has a second controllable valve for controlling“on-off” of the cleaning pipeline; the control device couples to thefirst controllable valve and the second controllable valve respectively,which controls the syringe to transfer the part of the first testingsample which is prepared by the blood sample and the first reagent fromthe reaction cell to the detection apparatus after the reaction betweenthe blood sample and the first reagent is finished, controls the firstcontrollable valve as “on” and the second controllable valve as “off”during the part of the first testing sample is drawn, and controls thefirst controllable valve off and the second controllable valve as “off”after drawing of the part of the first testing sample is finished; thecontrol device controls the first controllable valve as “off” and thesecond controllable valve as “on” after the leukocyte classificationdetection is finished, and controls the syringe to draw a cleaningsolution to clean the transfer pipeline and the detection apparatus anddischarge the cleaning solution from the cleaning pipeline; the controldevice controls the first controllable valve as “on” and the secondcontrollable valve as “off” after both the cleaning and the reactionbetween the reminder of the first testing sample and the second reagentare finished, and controls the syringe to transfer a part of the secondtesting sample through the transfer pipeline from the reaction cell tothe detection apparatus; and the control device controls the firstcontrollable valve as “off” and the second controllable valve as “off”after the drawing of the part of the second testing sample is finished.13. The blood analyzer according to claim 11, wherein said syringecomprises a first syringe and a second syringe, the sample injectingapparatus comprises a sample probe, and the transfer pipelinecomprising: a sample acquiring pipeline, which has a first end and asecond end, and the first end of the sample acquiring pipeline connectsto the sample probe; a first sample preparing pipeline, which has afirst end and a second end, and the first end of the first samplepreparing pipeline connects to the testing sample outlet of the reactioncell; a second sample preparing pipeline, which has a first end and asecond end, and the first end of the second sample preparing pipelineconnects to the second end of the first sample preparing pipeline andthe inlet of the detection apparatus; a first connecting pipeline, whichhas a first end and a second end, and the first end of the firstconnecting pipeline connects to the first syringe, the second end of thefirst connecting pipeline connects to an inlet for a diluent input; asecond connecting pipeline, which has a first end and a second end, andthe first end of the second connecting pipeline connects to the secondsyringe, the second end of the second connecting pipeline connects tothe second end of the sample acquiring pipeline and the second end ofthe second sample preparing pipeline respectively by a thirdcontrollable valve; an auxiliary pushing pipeline, which has a firstend, a second end and a fourth controllable valve, and the first end ofthe auxiliary pushing pipeline connects to the second connectingpipeline, the second end of the auxiliary pushing pipeline connects tothe first connecting pipeline; and a sheath fluid pipeline, which has afirst end, a second end and a fifth controllable valve, and the firstend of the sheath fluid pipeline connects to the first connectingpipeline, the second end of the sheath fluid pipeline connects to thedetection apparatus.
 14. The blood analyzer according to claim 10, saidblood analyzer further comprising a hemoglobin measurement apparatuswhich couples to the control device, and being controlled to measure thesecond testing sample for hemoglobin measurement while the reminder offirst testing sample reacts with the second reagent.